Engineering the Bacterial Laccase CotA for Functional Expression and Dye Decolorization Through Site-Directed Mutagenesis.

ABSTRACT: The relationship between the structure and function of bacterial laccases has garnered significant research attention thanks to their straightforward molecular structure. Nevertheless, studies examining the impact of an altered molecular structure on the heterologous expression of bacterial laccases in Escherichia coli remain scarce. Our research focuses on elucidating the impact of incorporating copper ions into the molecular structure of modified CotA on its exogenous expression in E. coli as well as its impact on the significance of the amino acid residues surrounding the internal electron channels and water molecule channels of the enzyme molecule. The results show that single-site mutation may affect the expression of CotA by affecting its soluble expression with differe

SUBMITTER: Zhou Z 

PROVIDER: S-EPMC12561958 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature