Genome-Wide Analysis and Functional Characterization of Small Heat Shock Proteins in <i>Allium sativum</i> L. Under Multiple Abiotic Stresses.

ABSTRACT: Small heat shock proteins play a pivotal role in maintaining protein homeostasis under abiotic stress conditions and are indispensable for plant viability. In the present study, a comprehensive characterization of this gene family in Allium sativum was conducted through genome-wide sequence identification, phylogenetic reconstruction, conserved motif analysis, promoter cis-element profiling, transcriptomic investigation, quantitative real-time PCR, subcellular localization, and yeast-based functional assays. A total of 114 small heat shock protein genes were identified across eight chromosomes and subsequently classified into ten phylogenetic subgroups. All encoded proteins conserved the α-crystallin domain, whereas their exon-intron architectures and promoter elements responsive to

SUBMITTER: Li N 

PROVIDER: S-EPMC12562054 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature