Precision Profiling of Disease Progression in Murine Models of Sepsis and Septic Shock.

ABSTRACT: Septic shock has an unacceptably high mortality rate and unmet need for new therapeutics. Murine models are crucial for research, yet methodologies often differ. This study characterised standard- and high-grade caecal ligation and puncture (CLP) murine models of septic shock by integrating ultraminiature arterial telemetry with comprehensive plasma biomarker analysis. Standard-grade and high-grade CLP was performed in 8-10 week old, male C57BL/6 mice (n = 98), with a subset implanted with arterial telemetry to monitor real-time circulatory function. Plasma markers of inflammation and organ damage were measured at multiple intervals up to 168 h post-CLP. Standard-grade and high-grade CLP showed distinct progressions; episodes of hypotension began 5-6 h after CLP in 30% of standard-grade an

SUBMITTER: Ramsay SD 

PROVIDER: S-EPMC12562319 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature