The Anti-Apoptotic Effect of C-Type Natriuretic Peptide and the Regulation of <i>NPPC</i> in Porcine Ovarian Granulosa Cells.

ABSTRACT: C-type natriuretic peptide (CNP), encoded by the NPPC (Natriuretic Peptide Precursor C), has been recognized as the principal endogenous factor sustaining oocyte meiotic arrest in mammalian follicles. Yet its influence on porcine ovarian granulosa cell fate and the regulatory mechanism of NPPC expression within these cells remain poorly understood. Here, utilizing an in vitro culture model of primary porcine ovarian granulosa cells and immature oocytes, we examined the impact of CNP on granulosa cell apoptosis and oocyte meiotic resumption, and elucidated the molecular circuitry governing NPPC expression. We found that follicular atresia in pigs was accompanied by a marked decline in the CNP receptor NPR2 (natriuretic peptide receptor 2). Correspondingly, exogenous CNP

SUBMITTER: Liu X 

PROVIDER: S-EPMC12562459 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature