Comparison of 3D culture systems on mesenchymal stem cell longevity, regenerative potential, and secretome production.

ABSTRACT:

Background/aims

Mesenchymal stem/stromal cells (MSCs) are widely investigated for regenerative therapies, yet current expansion methods often compromise their stem-like properties, limiting large-scale clinical translation. We aimed to evaluate whether a novel hydrogel-based Bio-Block® platform preserved intrinsic adipose-derived MSC (ASCs) phenotype and secretome compared with conventional systems.

Materials/methods

ASCs were cultured for four weeks in 2D, spheroids, Matrigel, or Bio-Blocks. Cultures were assessed for proliferation, senescence, apoptosis, trilineage differentiation, stem-like gene expression, secretome protein and extracellular vesicle (EV) production, and EV potency on endothelial cells (ECs).

Results

Bio-Block ASCs exhibited ~2-fold higher prolifer

SUBMITTER: Hodge J 

PROVIDER: S-EPMC12562725 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature