Optimising Extraction of Specific Food Allergens from Challenging Food Matrices for Immunoassay Quantification.

ABSTRACT: Simultaneous extraction of multiple clinically relevant, specific allergens from complex, processed foods for immunoassay quantification remains challenging. Moreover, shared extraction buffers remain elusive, which limits the effectiveness of multiplex (multi-target) technology. This study aimed to optimise extraction of specific allergens from challenging food samples and identify shared methods of extraction for multiplex analysis. Incurred processed food matrices (chocolate dessert, raw and baked biscuit) were prepared and extracted with 10 different buffers varying in base, pH, and additive content. Extracts were analysed by allergen-specific multiplex array and ELISA. Optimised recovery of 14 food allergens was obtained from complex incurred matrices using two extraction buffers: 50

SUBMITTER: Bermingham MD 

PROVIDER: S-EPMC12563046 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature