A Semi-Automatic Tool for the Standardized Analysis of Fluorescent Intensity Changes in Polarized Cells.

ABSTRACT: Imaging of intracellular messengers, like calcium, is one of the most reliable methods to follow real-time changes in several aspects of cellular activity, like receptor activation. However, the analysis could be influenced and biased by several factors like the location, shape, and size of the regions of interest (ROIs) and by the detection and correction of the movement of the preparation. Programs which are provided by the manufacturers are expensive and cannot be shared by collaborators. Many self-made programs have been implemented lately which have in-built cell recognizer ROI identification functions. These programs focus on the soma of the cells and neglect the processes, because in full tissue preparation finding cells is still challenging. Subcellular imaging experiments are stil

SUBMITTER: Fazekas F 

PROVIDER: S-EPMC12563114 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature