Development of KASP Molecular Markers and Candidate Gene Mining for Heat Tolerance-Related Traits in <i>Gossypium hirsutum</i>.

ABSTRACT: Background: High-temperature stress is one of the major abiotic stresses limiting cotton production. Identifying genetic loci and genes for heat tolerance is crucial for breeding heat-tolerant varieties. Methods: Given the complexity of heat tolerance phenotypes in cotton, this study, which focused on resource materials, identified an A/C SNP mutation at position 5486185 on chromosome D06 within the heat tolerance interval through genome-wide association studies (GWAS) of natural Gossypium hirsutum populations. Results: A total of 308 resource materials were identified and evaluated for their heat tolerance phenotypes over two years of field research. Kompetitive allele-specific PCR (KASP) molecular markers were developed on the basis of the D06-5486185 SNP to c

SUBMITTER: Gong Z 

PROVIDER: S-EPMC12563184 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature