Identification and Association of <i>CYP2R1, CYP27B1</i>, and <i>GC</i> Gene Polymorphisms with Vitamin D Deficiency in Apparently Healthy Population and in Silico Analysis of the Binding Pocket of Vitamin D3.

ABSTRACT: Vitamin D deficiency is highly prevalent in Pakistan, but there is limited data on its genetic aspects. This case-control pilot study aimed to determine the association of rs782153744, rs200183599, rs118204011, and rs28934604 with vitamin D deficiency along rs7041 which has been studied in our population. The DNA of a total of 600 subjects (300 cases and 300 controls) was extracted and genotyped by tetra ARMS PCR, followed by Sanger DNA sequencing of exon 4 of the CYP2R1 and CYP27B1 genes and exon 8 of the GC gene. SNP Stat was employed to analyze the data, while logistic regression was used to calculate the p-values and odds ratios (ORs). The R package version R studio (2025.05.1) Build 513 was used to statistically analyze rs782153744. In silico modeling of wi

SUBMITTER: Manzoor S 

PROVIDER: S-EPMC12563223 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature