Berberine Alleviates Intestinal Inflammation by Disrupting Pathological Macrophage-Epithelial Crosstalk in Macrophage-Organoid Co-Culture Model.

ABSTRACT: Berberine (BBR), a benzylisoquinoline alkaloid isolated from Chinese herb Coptis chinensis, has been widely used clinically to treat intestinal infectious diseases. Recently, it has been found to have multiple pharmacological effects, including anti-inflammatory activity and immune effects in inflammatory bowel disease (IBD). However, its exact targets remain to be elucidated. In this study, we used a mouse intestinal organoid-macrophage co-culture model to investigate the anti-inflammatory effects and immune effects of BBR. Our findings demonstrated that lipopolysaccharide (LPS) induced more robust inflammatory responses and epithelium damage in the co-culture system compared to the organoid alone. BBR effectively attenuated inflammation and restored epithelial barrier integrity by

SUBMITTER: Han Y 

PROVIDER: S-EPMC12563409 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature