Developing Endogenous Autophagy Reporters in <i>Caenorhabditis elegans</i> to Monitor Basal and Starvation-Induced Autophagy.

ABSTRACT: Autophagy (cellular self-eating) is a tightly regulated catabolic process of eukaryotic cells during which parts of the cytoplasm are sequestered and subsequently delivered into lysosomes for degradation by acidic hydrolases. This process is central to maintaining cellular homeostasis, the removal of aged or damaged organelles, and the elimination of intracellular pathogens. The nematode Caenorhabditis elegans has proven to be a powerful genetic model for investigating the regulation and mechanism of autophagy. To date, the fluorescent autophagy reporters developed in this organism have predominantly relied on multi-copy, randomly integrated transgenes. As a result, the interpretation of autophagy dynamics in these models has required considerable caution due to possible overexpress

SUBMITTER: Borden K 

PROVIDER: S-EPMC12563416 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature