Changes in neural progenitor lineage composition during astrocytic differentiation of human iPSCs.

ABSTRACT: The regional specificity of stem cell-derived astrocytes is believed to be an important prerequisite for their application in disease modelling and cell-based therapies. Due to the lack of subtype-defining markers for astrocytes in different regions of the brain, the regional identity of in vitro-derived astrocytes is often declared by the dominant positional characteristics of their antecedent neural progenitors, patterned to a fate of interest, with the assumption that the positional trait is preserved by the derived astrocytes via linear descent. Using a human induced pluripotent stem cell line designed for tracing derivatives of LMX1A⁺ cells combined with a ventral midbrain induction paradigm, we show that astrocytes originating from LMX1A⁺ progenitors can only be

SUBMITTER: Li Z 

PROVIDER: S-EPMC12563561 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature