Age-Dependent Redistribution of the Life-Important Enzyme in the Retina: Adult Muller Glial Cells' Endfeet Lack Spermine Synthase Expression.

ABSTRACT: Polyamine (PA) spermine (SPM) (i) plays an essential role in the function of neurons, while (ii) accumulating predominantly in glial cells by an unknown mechanism. In addition, the translocation of SPM synthesis and redistribution in the developing and maturating retinas remains unclear. Therefore, the expression of the SPM-synthesizing enzyme, spermine synthase (SpmS), was compared in rat retinas on postnatal days 3, 21, and 120 using immunocytochemistry, Western blot (WB), and ImageJ analyses. The anti-glutamine synthetase (GS) antibody identified glial cells, and DAPI labeled the cell nuclei. At postnatal day 3 (P3), the neonatal retina shows widespread SpmS expression throughout most neuroblast cells, but absent in the developing synaptic layers and Müller cell (MCs) processes. By day

SUBMITTER: Zayas-Santiago A 

PROVIDER: S-EPMC12563582 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature