House Dust Mite Nebulization Drives Alarmin and Complement Activation in a Murine Tracheal Air-Liquid Interface Culture System.

ABSTRACT: Air-liquid interface (ALI) cultures offer a physiologically relevant in vitro model of the airway epithelium (AE), capable of recapitulating key structural and functional features observed in vivo. In this study, we established and validated a murine ALI culture system comprising pseudostratified epithelia with functional tight junctions, ciliated cells and goblet cells. To assess their innate immune functions, we designed and 3D-printed an autoclavable aerosol deposition chamber, which allowed us to expose differentiated AE cultures to house dust mite (HDM) allergen. Upon HDM exposure, AE cells mounted a time-dependent innate immune response characterized by the secretion of complement component C3, the generation of its active cleavage products C3a and increased expression of C3aR and C5

SUBMITTER: Ahmad JH 

PROVIDER: S-EPMC12563611 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature