Rhythmic Dynamics of Stress Granules in <i>Wild-Type</i> and <i>Bmal1^-/-</i> Fibroblasts Lacking a Functional Canonical Circadian Clock.

ABSTRACT: Circadian rhythms are endogenous ~24 h oscillations that regulate diverse biochemical processes. Although stress responses can exhibit circadian modulation, evidence for rhythmic regulation of stress granules (SGs)-cytoplasmic RNA-protein condensates formed under stress-remains limited. We investigated sodium arsenite-induced SG dynamics in NIH/3T3 cultures. SG number, eIF3 signal intensity-an established SG marker-and area oscillated with a period of ~24 h. These rhythms persisted in Bmal1^-/- mouse embryonic fibroblasts (MEFs), despite lacking a transcription-translation feedback loop (TTFL) that constitutes the canonical circadian clock, but with altered amplitude and phase, indicating partial dependence on the molecular clock. Several SG-associated RNA-binding proteins

SUBMITTER: Malcolm M 

PROVIDER: S-EPMC12564134 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature