RNAi of <i>vATPasea</i> Affects Survival and Larval-Pupal Development in <i>Plutella xylostella</i>.

ABSTRACT: The diamondback moth, Plutella xylostella, is a major pest of brassica vegetables and oilseed crops, posing a serious threat to China's grain and oil production. RNA interference (RNAi) has been developed as an efficient strategy to control pests. In this study, the effects of RNAi on P. xylostella were evaluated by injecting two doses of synthesized dsPxvATPasea. The transcripts of PxvATPasea were widely transcribed during different developmental stages from egg to adult. They were abundantly expressed in the hindgut and Malpighian tubules, compared with other tissue types. Introduction of 800 ng dsPxvATPasea in the fourth-instar larvae greatly reduced corresponding mRNA levels by 3.1 and 1.4 times on day 2 and 3, respectively, causing 66.6% mortality an

SUBMITTER: Yu X 

PROVIDER: S-EPMC12564257 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature