Development of a PCR Assay for the Detection of <i>Legionella micdadei</i> in the Environment.

ABSTRACT:

Background/objectives

Legionella micdadei is a clinically significant species within the Legionella genus, requiring accurate detection methods, surveillance, and precise clinical diagnosis. Our objective was to develop a sensitive polymerase chain reaction (PCR) assay specific for L. micdadei to detect its presence in environmental specimens.

Methods

We targeted the 23S-5S intergenic spacer region, which can differentiate Legionella spp. We tested the detection of L. micdadei with 20 strains and determined the limit of detection with 2 strains. We verified assay specificity with 17 strains of other Legionella spp., 62 strains of other bacterial and fungal genera, and three human DNA specimens. We evaluated intra- and inter-run preci

SUBMITTER: Belanger WN 

PROVIDER: S-EPMC12564405 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature