Stability Matters: Revealing Causal Roles of G-Quadruplexes (G4s) in Regulation of Chromatin and Transcription.

ABSTRACT:

Background

G-quadruplexes (G4s) are non-canonical higher-order nucleic acid structures that form at guanine-rich motifs, with features spanning both secondary and tertiary structural levels. These dynamic structures play pivotal roles in diverse cellular processes. Endogenous G4s (eG4s) function through their dynamically formed structures, prompting the hypothesis that their thermostability, as a key structural property, may critically influence their functionality. This study investigates the relationship between G4 stability and other functional genomic signals within eG4 regions and examines its broader impact on chromatin organization and transcriptional regulation.

Methods

We developed a mapping strategy to associate in vitro-derived thermostability metrics and multi-om

SUBMITTER: Xiao K 

PROVIDER: S-EPMC12564469 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature