The Role of miR-326-3p in Regulating Differentiation and Thermogenesis Genes in Goat Brown Adipocytes.

ABSTRACT: Background: Brown adipose tissue (BAT) is indispensable for producing heat and contributes critically to the survival of neonatal mammals. MicroRNAs (miRNAs) are small noncoding RNAs that serve as key post-transcriptional regulators, playing a crucial role in regulating BAT development and thermogenesis. However, the role of miR-326-3p in goat brown adipocytes remains largely unclear. Methods: Primary brown adipocytes were isolated from goat perirenal adipose tissue and subjected to gain and loss-of-function assays using miR-326-3p mimics and inhibitors. Lipid accumulation, thermogenic-related genes, and mitochondrial gene expression were quantified by Oil Red O staining and qRT-PCR. Target prediction and dual-luciferase reporter assays were performed to validate direct inter

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC12564603 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature