AAV2retro Enters Axons of Passage and Extensively Transduces Corticospinal Neurons After Injection into Spinal White Matter.

ABSTRACT:

Background

Adult neurons in the central nervous system often fail to regenerate after spinal cord injury (SCI). Regenerative gene therapies could potentially promote corticospinal axon regeneration, restoration of motor circuitry, and functional improvement after SCI, but translational methods for targeted gene delivery to corticospinal neurons are needed. AAV2retro is an engineered variant of the adeno-associated virus 2 (AAV2) capsid that demonstrates greatly enhanced retrograde transduction of projection neurons. When injected into spinal gray matter, AAV2retro retrogradely transduces neurons in the sensorimotor cortex that project to the injected spinal level.

Methods

We initially hypothesized that injection of AAV2retro into the dorsal column white matter immediately ro

SUBMITTER: Nakashima KT 

PROVIDER: S-EPMC12564753 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature