Application of Modulators of Ca²⁺-Activated Big-Conductance Potassium Channels Against Cd²⁺-Induced Cytotoxicity: A Study on Two Rat Cell Lines, PC12 and AS-30D.

ABSTRACT: As we found earlier, paxilline (a Penicillium paxilli mycotoxin and blocker of Ca²⁺-activated big-conductance potassium channels, BK(Ca)s) attenuated Cd²⁺-induced cytotoxic effects, whereas BK(Ca) activators (NS004, NS1619) and Cd²⁺ were able to induce apoptosis, which was enhanced when used together. In this work, molecular mechanisms underlying the aforementioned effects were studied using two rat cell lines, PC12 and AS-30D, flow cytometry, and spectrofluorometric and polarographic techniques. Both NS004 and NS1619 were found to have time- and dose-dependent effects on cell viability, respiration, mitochondrial membrane potential, and intracellular reactive oxygen species (ROS) production. In PC12 cells, BK(Ca) openers exerted an uncoupling effect after

SUBMITTER: Belyaeva EA 

PROVIDER: S-EPMC12564761 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature