Silencing the <i>cyp314a1</i> and <i>cyp315a1</i> Genes in the <i>Aedes albopictus</i> 20E Synthetic Pathway for Mosquito Control and Assessing Algal Blooms Induced by Recombinant RNAi <i>Microalgae</i>.

ABSTRACT: As one of the key vectors for the transmission of Dengue fever, Aedes albopictus is highly ecologically adaptable. The development of environmentally compatible biological defence and control technologies has therefore become an urgent need for vector biological control worldwide. This study constructed and used double-stranded RNA (dsRNA) expression vectors targeting the cyp314a1 and cyp315a1 genes of Ae. albopictus to transform Chlamydomonas reinhardtii and Chlorella vulgaris, achieving RNA interference (RNAi)-mediated gene silencing. The efficacy of the RNAi recombinant algal strain biocide against Ae. albopictus was evaluated by administering it to Ae. albopictus larvae. The results showed that the oral administration of the cy

SUBMITTER: Deng X 

PROVIDER: S-EPMC12564949 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature