Effects of Temperature, pH, and Relative Humidity on Growth of <i>Penicillium crustosum</i> OM1 Isolated from Pears and Its Penitrem A Production.

ABSTRACT: Penitrem A, an indole-diterpenoid neurotoxin, is produced by several species of Penicillium on cereal grains, meat, dairy products, and fruits. Penicillium crustosum is a well-known penitrem A producer, but much is unknown about physiological characteristics of P. crustosum. In this study, we isolated penitrem A-producing P. crustosum OM1 from pears, and investigated the influence of temperature, pH, and relative humidity [RH] on its growth and penitrem A production. The fungal species exhibited the highest growth at 25 °C and pH 4.5 on mYES4 under RH 98%, whereas it produced the highest level of penitrem A at 22 °C and pH 6.5 on the same media under RH 98%. Furthermore, RT-qPCR analysis of six penitrem A biosynthetic genes (ptmB, ptmJ, ptmK

SUBMITTER: Gao S 

PROVIDER: S-EPMC12564995 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature