Rapid Detection of <i>Philaenus italosignus</i> Drosopoulos & Remane, 2000 (Hemiptera: Aphrophoridae) with Real-Time PCR Probe LNA Technology.

ABSTRACT: To date, Philaenus spumarius (Linnaeus, 1758), Philaenus italosignus Drosopoulos & Remane, 2000, and Neophilaenus campestris (Fallén, 1805) are proven vectors of the phytopathogenic bacterium Xylella fastidiosa Wells et al., 1987 in Europe. Currently, the identification of these three species relies on the well-documented status of morphological and taxonomical characters, making the discrimination of vector adult males possible by genitalia comparison. This study updates the biomolecular diagnostic tests with a rapid identification tool for P. italosignus, using locked nucleic acid (LNA) probe technology. The test also overcomes the difficulties associated with the morphological identification of females and juveniles. The morphological α-taxonomic ident

SUBMITTER: Rizzo D 

PROVIDER: S-EPMC12565103 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature