Sustainable and Integral Valorization of <i>Dosidicus gigas</i> Pen Waste: Combined Production of Chitosan with Antibacterial Properties and Human and Marine Probiotics.

ABSTRACT: This study details a biorefinery approach to valorize Dosidicus gigas squid pen waste. The process starts with the enzymatic deproteinization of squid pens, which prove effective with both Alcalase and Novozym, with the latter exhibiting a slightly higher efficiency to yield a material with 73% chitin content. Subsequent alkaline hydrolysis produces highly deacetylated chitosan (>90% degree of deacetylation), followed by controlled depolymerization to obtain polymers with molecular weights ranging from 50 to 251 kDa. Both native and depolymerized chitosan exhibit antimicrobial activity against Escherichia coli and Bacillus cereus, with B. cereus demonstrating greater resistance to chitosan compared to E. coli. The research also explores the bioconversion

SUBMITTER: Lima M 

PROVIDER: S-EPMC12565315 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature