Computational-Experimental Identification of Palindromic Motifs Bound by Bacterial XRE Family Transcriptional Regulators.

ABSTRACT: Bacteria employ transcriptional regulators, such as those belonging to the Xenobiotic Response Element (XRE) family, to regulate metabolic processes. These regulators often exhibit autoregulatory properties and function as dimers to recognize palindromic DNA motifs. However, the binding motifs of the XRE family transcriptional regulators in bacteria have not yet been well characterized on a large scale. To identify potential XRE transcriptional regulator recognition motifs efficiently, we developed a computational approach combining structural alignment, sequence scanning, and motif clustering. We first identified the potential motifs of XRE regulators using computational methods. Using the helix-turn-helix (HTH) domain of XRE family regulators as a template, we collected 27,732 proteins c

SUBMITTER: Wang L 

PROVIDER: S-EPMC12565409 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature