Improving the Biocompatibility of Plant-Derived Scaffolds for Tissue Engineering Using Heat Treatment.

ABSTRACT: Small-diameter vascular grafts often fail due to thrombosis and compliance mismatch. Decellularized plant scaffolds are a biocompatible, sustainable alternative. Leatherleaf viburnum leaves provide natural architecture and mechanical integrity suitable for tissue-engineered vessels. However, the persistence of immunogenic plant biomolecules and limited degradability remain barriers to clinical use. This study tested whether mild heat treatment improves scaffold biocompatibility without compromising mechanical performance. Decellularized leatherleaf viburnum scaffolds were treated at 30-40 °C in 5% NaOH for 15-60 min and then evaluated via tensile testing, burst pressure analysis, scanning electron microscopy, histology, and in vitro assays with white blood cells and endothelial cells. Scaf

SUBMITTER: Ramsamooj A 

PROVIDER: S-EPMC12565551 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature