Virulence and Stress-Related Proteins Are Differentially Enriched and <i>N</i>-Terminally Acetylated in Extracellular Vesicles from Virulent <i>Paracoccidioides brasiliensis</i>.

ABSTRACT: Extracellular vesicles (EVs) are bilayer-membrane cellular components that deliver protected cargo to the extracellular environment and can mediate long-distance signaling. We have previously reported that EVs isolated from the virulent fungal pathogen Paracoccidioides brasiliensis Vpb18 can revert the expression, in the attenuated variant Apb18, of stress-related virulence traits. We presently show that the Vev and Aev, respectively, produced by these variants display distinct proteomes, with prevalent functional enrichment in Vev related to oxidative stress response, signal transduction, transport, and localization, in addition to richer protein-protein interaction. Proteome sequences were obtained by nanoflow liquid chromatography coupled with tandem mass spectrometry (nano LC-ES

SUBMITTER: Octaviano-Azevedo CE 

PROVIDER: S-EPMC12565617 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature