Diagnostic Fragmentations of Animal and Fungal Sterols/Stanols Obtained by APCI-Tandem Mass Spectrometry: A Route Towards Unknown Free Sterol Identification.

ABSTRACT: Background/Objectives: Animal and fungal sterols and stanols exhibit remarkable structural diversity, driven by variations in the number and position of C=C bonds within the steroidal tetracyclic core and side chain, along with diverse branching patterns of the latter. Similarly to phytosterols, these metabolites produce highly complex tandem mass spectra, whose interpretation has so far been limited. To address this gap, the fragmentation behavior of selected animal/fungal sterols and stanols was studied in this paper. Methods: Higher-Collisional-energy Dissociation-High-resolution tandem mass spectrometry (HCD-HRMS/MS) of protonated/dehydrated species generated via atmospheric pressure chemical ionization (APCI) was performed on structurally diverse compounds, including lat

SUBMITTER: Cinquepalmi V 

PROVIDER: S-EPMC12565904 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature