Accelerated Screening of Wheat Gluten Strength Using Dual Physicochemical Tests in Diverse Breeding Lines.

ABSTRACT: Introducing fast, reliable, and low-input technologies that utilize wholemeal wheat is essential for efficiently screening gluten quality in wheat breeding lines. Although the GlutoPeak Tester (GPT) has been widely studied for gluten assessment, its application in breeding programs remains underexplored. This study presents a comprehensive approach to optimizing a GPT protocol using a diverse set of genotypes collected over seven harvest years and multiple environments. To improve screening capabilities, a quick and simple protein fractionation (PF) technique was integrated into the workflow. Key GPT parameters-such as peak maximum time, maximum torque, and aggregation energy-along with the newly proposed PM-AM parameter, showed strong correlations with established quality traits. PF data,

SUBMITTER: Hadinezhad M 

PROVIDER: S-EPMC12566020 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature