Comparison of Next-Generation Sequencing, Real-Time PCR and HRM-PCR for <i>Helicobacter pylori</i> Detection in Pediatric Biopsies.

ABSTRACT: In 40 unique pediatric biopsy samples, this study aimed to compare the obtained results of Helicobacter pylori DNA detection using next-generation sequencing (NGS), a real-time PCR-based IVD-certified kit and an established high resolution melting real-time PCR-based method for H. pylori-specific ureA gene. From the same group, the H. pylori DNA was identified in 16 (40.0%) samples in both real-time PCR-based methods, with quantification cycle (Cq) values ranging from 17.51 to 32.21 for the IVD kit. NGS was able to detect H. pylori DNA in 14 (35.0%) samples, with read counts between 7768 and 42,924. While all three methods showed similar detection rates, both PCR variants were slightly more sensitive, identifying H. pylori in two additional samples

SUBMITTER: Bogiel T 

PROVIDER: S-EPMC12566025 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature