<i>Legionella pneumophila</i>-Induced NETs Do Not Bear LL-37 Peptides.

ABSTRACT: Legionella pneumophila (L. pneumophila) infection is characterized by a wide spectrum of manifestations, from influenza-like illness to life-threatening atypical pneumonia with multiorgan failure. The aim of our study was the assessment of in vitro and ex vivo neutrophil activation in L. pneumophila infections, as well as the role of neutrophils' peptides such as LL-37 in infection. The ability of neutrophils to form ex vivo extracellular traps (NETs) in response to bacterial infection was examined by immunofluorescence. In parallel, patients' sera, as well as opsonized standard L. pneumophila strains, were used for in vitro activation of neutrophils from healthy individuals. The serum levels of interleukins were assessed using the LEGENDplexTM Multi-Analyte Flo

SUBMITTER: Iliadi V 

PROVIDER: S-EPMC12566110 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature