Microbiological Safety of Donor Human Milk: Comparing Culture-Based Methods for Enterobacterales Detection.

ABSTRACT: In neonatal care, donor human milk (DHM) is used when maternal milk is unavailable or insufficient. In several countries, including Germany, raw (i.e., unpasteurised) DHM is occasionally administered under specific clinical conditions. However, the lack of standardised, evidence-based microbiological testing protocols raises concerns about the reliability of safety assessments for this high-risk patient group. The objective of this study was to assess the performance of four culture-based microbiological methods for detecting Enterobacterales in donor human milk, using both spiked samples and raw milk. We compared the detection limits of four culture-based microbiological methods, with and without enrichment, using spiked DHM samples and 93 raw DHM samples from a single donor (limited gene

SUBMITTER: Dawczynski L 

PROVIDER: S-EPMC12566355 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature