Antioxidant Action of Dinitrosyl Iron Complexes in Model Systems Containing Cytochrome <i>c</i> and Organic Hydroperoxides.

ABSTRACT: The antioxidant/prooxidant effects of dinitrosyl iron complexes (DNICs), physiological donors of nitric oxide (NO^•), are studied in reaction systems modeling processes with cytochrome c occurring in mitochondria under oxidative stress and leading to apoptosis. Using luminol-dependent chemiluminescence, DNICs with glutathione and phosphate ligands were shown to decrease the level of prooxidants in a reaction system containing ferricytochrome c and cumene hydroperoxide. Electron paramagnetic resonance (EPR) spectroscopy revealed that glutathione DNICs (DNICs-GS) intercepted the free radicals formed during the interaction between cytochrome c and tert-butyl hydroperoxide. DNICs-GS were also shown to prevent the formation of oligomeric forms of cytochrome

SUBMITTER: Kosmachevskaya OV 

PROVIDER: S-EPMC12566574 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature