Effects of Different Centrifugation Parameters on Equilibrium Solubility Measurements.

ABSTRACT: The bioavailability of a drug is closely linked to its solubility, making its early determination essential in drug development. The saturation shake-flask (SSF) method is the gold standard protocol for this, which includes a phase separation step-either by sedimentation, filtration, or centrifugation. This step is critical, as it can directly influence the accuracy of the results. This study investigated the impact of centrifugation parameters-time and rotation speed-on solubility measurements. Additionally, we compared two sample preparation protocols: continuous stirring for 24 h versus 6 h of stirring followed by 18 h of sedimentation before centrifugation. Four model compounds were tested at three pH values using Britton-Robinson buffers. Centrifugation was conducted for 5, 10, or 20

SUBMITTER: Szollath R 

PROVIDER: S-EPMC12566593 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature