Developmental Regulation of the Murine Selenoproteome Across Embryonic and Postnatal Stages: Implications for Human Nutrition and Health.

ABSTRACT: Background/Objectives: Selenoproteins play indispensable roles in embryonic development, with their dysregulation linked to various metabolic and neurological disorders. This study aims to systematically quantify the mRNA expression levels of all 24 selenoprotein genes in murine heart, brain, liver, and kidney tissues across embryonic (E8.5, E12.5, E18.5) and postnatal (P7, P30, P90) developmental stages, in order to elucidate the regulatory landscape of selenium metabolism during development. Methods: We collected tissues from mice at six developmental stages and performed RNA extraction followed by quantitative real-time PCR (qPCR) to measure the expression of all 24 selenoprotein genes. Data were normalized using the geometric mean of ActB and Gapdh, and statistical analys

SUBMITTER: Wang SS 

PROVIDER: S-EPMC12566671 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature