Evaluation of Suitable Reference Gene During the Development of Paired or Unpaired Female <i>Schistosoma japonicum</i> on the 18th and the 23rd Days Post Infection.

ABSTRACT:

Background

Identifying optimal housekeeping genes is essential to accurately quantify gene expression dynamics across the 18th day (male and female begin to pair) and the 23rd day (female begin to sex mature) post infection of Schistosoma japonicum, because this process involves selecting suitable housekeeping genes to ensure the reliability and accuracy of all subsequent expression analyses, thereby improving the precision of biological interpretations. Schistosoma japonicum transcriptomics reveals marked stage-dependent variation in candidate reference genes, which directly challenges the long-standing hypothesis that commonly recommended reference genes remain stably expressed throughout the 18th day and the 23rd day post-infection developmental phases and therefor

SUBMITTER: Wang S 

PROVIDER: S-EPMC12566883 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature