Overexpression of VtF3'5'H and RhNHX Genes Alters Flower Color and Plant Morphology in Transgenic Rose 'Red Farm'.

ABSTRACT: Roses (Rosa hybrida) are among the most highly valued ornamental plants worldwide, with flower color serving as a major determinant of consumer preference and commercial success. However, the absence of the flavonoid 3',5'-hydroxylase (F3'5'H) gene limits delphinidin biosynthesis, making it difficult to achieve blue or purple pigmentation. Vacuolar sodium/proton antiporters (NHX) regulate vacuolar pH and are also implicated in color stability. In this study, we introduced Viola tricolor F3'5'H (VtF3'5'H) and Rosa hybrida NHX (RhNHX) into the rose cultivar 'Red Farm' using Agrobacterium-mediated transformation. The non-native VtF3'5'H gene was detected in transgenic plants but not in the wild type, while RhNHX expression was rel

SUBMITTER: Lee KY 

PROVIDER: S-EPMC12567232 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature