Interplay of the Mass Transport and Reaction Kinetics for Lateral Flow Immunoassay Integrated on Lab-on-Disc.

ABSTRACT: Lateral Flow Assays (LFAs) are ubiquitous test platforms due to their affordability and simplicity but are often limited by low sensitivity and lack of flow control. The present work demonstrates the combination of LFAs with centrifugal microfluidic platforms that allows for enhancement of LFAs' sensitivity via the increase in the dwell time of the analyte at the test line as well as by passing a larger sample volume through the LFA strip. The rate of advancement of the liquid front in the radially positioned NC strip is retarded by the centrifugal force generated on spinning disc; therefore, the dwell time of the liquid front above the test line of LFA is increased. Additionally, integrating a waste reservoir enables passive replenishment of additional sample volume increases total probed

SUBMITTER: Peshin S 

PROVIDER: S-EPMC12567277 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature