An Integrated Approach for the Comprehensive Characterization of Metabolites in Broccoli (<i>Brassica oleracea</i> var. <i>Italica</i>) by Liquid Chromatography High-Resolution Tandem Mass Spectrometry.

ABSTRACT:

Background

Broccoli contains diverse phytochemicals, including glucosinolates and their hydrolysis products, with potential nutritional and bioactive properties. Accurate metabolite profiling requires optimized sample preparation and comprehensive databases.

Methods

A rapid enzymatic deactivation method with 70% methanol, implemented prior to cryogrinding, was evaluated for processing freeze-dried and fresh broccoli florets, which were compared as plant materials. A widely targeted, organ-resolved metabolite database was constructed by integrating over 612 reported phytochemicals with glucosinolate degradation products. LC-HRMS combined with MS-DIAL and GNPS was employed for metabolite detection and annotation.

Results

Freeze-dried samples yielded nearly twice the num

SUBMITTER: Hu Z 

PROVIDER: S-EPMC12567488 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature