A Novel Flow Cytometry Array for High Throughput Detection of SARS-CoV-2 Antibodies.

ABSTRACT:

Background/objectives

Although the U.S. Food and Drug Administration (FDA) has approved one antiviral treatment and authorized others for emergency use, there is no fully effective antiviral therapy for coronavirus disease 2019 (COVID-19), which is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Assays detecting virus-specific immunoglobulins (Ig) or nucleic acids in large-scale epidemiological, vaccine, and drug development studies remain limited due to high costs, reagent accessibility, and cumbersome protocols.

Methods

A multiplex bead-based assay was developed to simultaneously detect human IgM, IgG, and IgA antibodies against the SARS-CoV-2 spike receptor binding domain (RBD) in serum using flow cytometry. Assay performance was evaluated for

SUBMITTER: Zhang B 

PROVIDER: S-EPMC12567774 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature