Effect of Oxygen Tension Modification During Oocyte Maturation on Porcine Oocyte Quality.

ABSTRACT: This study investigated the effects of high (atmospheric) and low (5% O₂) oxygen tension, as well as a combination of the two, on oocyte metabolism and quality during maturation. Cumulus cell-oocyte complexes collected from gilt ovaries were used for in vitro maturation. In addition, RNA-seq was conducted on the cumulus cells. Low oxygen tension throughout oocyte maturation did not alter the developmental rate to the blastocyst stage; however, it increased oocyte ATP and lipid content while reducing mitochondrial reactive oxygen species and mitochondrial membrane potential. Low-oxygen conditions increased glucose consumption but reduced mitochondrial DNA copy number and mitochondrial protein in cumulus cells. RNA-seq of cumulus cells revealed that low oxygen tension reduced mito

SUBMITTER: Inoue Y 

PROVIDER: S-EPMC12568154 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature