Absorption and Tissue Distribution of Environmental Pollutant HFPO-DA, and Its Effect on Hepatic Lipid Metabolism Reprogramming in Mice.

ABSTRACT:

Objective

Hexafluoropropylene oxide dimer acid (HFPO-DA), also known as GenX, is widely used globally, raising concerns about its safety and public health implications. However, its toxicity mechanism remains unclear. The purpose of this study was to develop a reliable method for detecting HFPO-DA in mice and to investigate its absorption, distribution, and impact on hepatic lipid metabolism.

Method

HFPO-DA levels were measured in the serum and eight tissues of C57BL/6J mice after oral administration using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Lipid metabolites in the liver were also detected and analyzed.

Results

HFPO-DA was rapidly absorbed into the bloodstream and widely distributed throughout all tested tissues. It penetrat

SUBMITTER: Peng J 

PROVIDER: S-EPMC12568254 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature