Robust inhibitory glycinergic transmission and the effect of bafilomycin, folimycin and EIPA: lessons from the auditory brainstem.

ABSTRACT: Sustained synaptic transmission requires the continuous replenishment of released synaptic vesicles (SVs). This process is particularly critical in neuronal circuits that operate at high rates and with high temporal precision, such as those in the auditory brainstem. Here, we investigated the effect of SV (re-)filling on inhibitory synapses between the medial nucleus of the trapezoid body (MNTB) and the lateral superior olive (LSO). These synapses transmit information with high speed and fidelity, properties essential for auditory computations such as sound localization. We specifically examined the role of the vacuolar ATPase (V-ATPase), a proton pump that acidifies the SV lumen to enable neurotransmitter loading. Using patch-clamp recordings in acute mouse slices, we assessed synaptic fu

SUBMITTER: Pizzi E 

PROVIDER: S-EPMC12568497 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature