Microbial production and functional assessment of <i>γ</i>-polyglutamic acid isolated from <i>Bacillus</i> sp. M-E6.

ABSTRACT: This study describes the production and functional evaluation of γ-polyglutamic acid (γ-PGA) synthesized by Bacillus sp. M-E6, isolated from a traditional fermented food. Among 103 isolates screened, strain Bacillus sp. M-E6 exhibited the highest γ-PGA yield (4.9 g/L) and was taxonomically identified through 16S rRNA gene sequencing. The γ-PGA was extracted and characterized for the physicochemical and techno-functional properties. The γ-PGA was purified using ion-exchange column chromatography, and its molecular mass was determined by Electrospray Ionization-Mass Spectrometry (ESI-MS), which found it to be 60.42 kDa. Fourier-transform infrared spectroscopy and nuclear magnetic resonance analysis confirmed the polymer's structure as γ

SUBMITTER: Manika V 

PROVIDER: S-EPMC12568500 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature