Galactin-3 regulation of CDC42 promotes neuronal autophagy following spinal cord injury.

ABSTRACT:

Background

Spinal cord injury (SCI) is a debilitating condition within the nervous system with a high disability rate and substantial economic burden. The functional recovery following SCI is enhanced by moderate levels of autophagy but hindered when autophagy becomes excessive. Galectin-3 (GAL3) has been recognized as an autophagy regulator; however, its role in SCI and its associated mechanism are largely unknown.

Methods

The Walsh clamping method was employed to establish a rat SCI model, while a high-concentration glutamate incubation method was used to create an in vitro model of spinal cord neuronal injury. Subsequent to establishing the injury models, the expression levels of GAL3 were detected using QPCR and Western Blot. Immunohistochemical staining was perfo

SUBMITTER: Yan L 

PROVIDER: S-EPMC12568528 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature