Mitochondrial genome of <i>Isatis indigotica</i> reveals repeat-mediated recombination and phylogenetic insights in <i>Cruciferae</i>.

ABSTRACT: Isatis indigotica is an important medicinal plant extensively used in traditional Chinese medicine for its antiviral and anti-inflammatory properties. While previous studies have elucidated its nuclear and plastid genomes, the mitochondrial genome (mitogenome) -critical for understanding organellar evolution, intracellular DNA transfer, and stress response mechanisms-has remained uncharacterized. Here, we present a complete de novo assembly and comprehensive analysis of the I. indigotica mitogenome, generated using high-fidelity long-read sequencing technologies. The circular mitogenome spans 260,864 bp and encodes 31 protein-coding genes, 21 transfer RNAs, and 3 ribosomal RNAs. Repetitive sequences constitute 12.3% of the genome, with large repeats mediating homologou

SUBMITTER: Yu S 

PROVIDER: S-EPMC12568568 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature