Prior exposure strongly influences mechanisms underpinning survival of heat shock in <i>Escherichia coli</i>.

ABSTRACT: The heat shock response of Escherichia coli represents a canonical example of how bacteria can recognize a stress and invoke a protective response by altering specific gene regulation. However, most understanding of the processes involved arises from experiments where cells have been subjected to immediate heat shock. In this study, we identified the populations of transposon mutants in E. coli BW25113 involved in response to sudden heat shock and stepwise heat stress conditions. We used Transposon-Directed Insertion Site Sequencing with expression (TraDIS-Xpress) to identify genes whose function or expression contributed to survival under 5 different heat conditions. These conditions included direct exposure to 44°C, 47°C, or 50° C referred to as "heat shock" or half

SUBMITTER: Yasir M 

PROVIDER: S-EPMC12568643 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature