Identification of dominant peptide epitopes and antibody response to the spike protein of SARS-CoV-2.

ABSTRACT:

Objective

Multiple antigenic epitopes are present in the spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but they exhibit significant variation in immunogenicity and reactogenicity. We aimed to investigate the reactivity of different SARS-CoV-2 antigenic epitopes with serum antibodies from patients with COVID-19 and vaccinated individuals to characterize specific antibody responses.

Methods

Linear B-cell peptides derived from the SARS-CoV-2 S protein were screened using the Immune Epitope Database (IEDB). These linear peptides were coupled to bovine serum albumin (BSA), and blot hybridization was used to investigate antigen-antibody reactions among the peptides, commercial anti-S protein polyclonal antibodies, and sera derived from vaccinat

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12568650 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature