Drosophila ELL is associated with actively elongating RNA polymerase II on transcriptionally active sites in vivo.

ABSTRACT: Several factors have been biochemically characterized based on their ability to increase the overall rate of transcription elongation catalyzed by the multiprotein complex RNA polymerase II (Pol II). Among these, the ELL family of elongation factors has been shown to increase the catalytic rate of transcription elongation in vitro by suppressing transient pausing. Several fundamental biological aspects of this class of elongation factors are not known. We have cloned the Drosophila homolog (dELL) in order to test whether ELL family proteins are actually associated with the elongating Pol II in vivo. Here we report that dELL is a nuclear protein, which, like its mammalian homologs, can increase the catalytic rate of transcription elongation by Pol II in vitro. Interestingly, we find that dE

SUBMITTER: Gerber M 

PROVIDER: S-EPMC125687 | biostudies-literature | 2001 Nov

REPOSITORIES: biostudies-literature